Prostaglandin F2α, progesterone and oxytocin production by cultured bovine luteal cells treated with prostaglandin E2 and pregnancy-specific protein B
Identifieur interne : 003F51 ( Main/Exploration ); précédent : 003F50; suivant : 003F52Prostaglandin F2α, progesterone and oxytocin production by cultured bovine luteal cells treated with prostaglandin E2 and pregnancy-specific protein B
Auteurs : R. P. Del Vecchio [Canada] ; W. D. Sutherland [Canada] ; R. G. Sasser [États-Unis]Source :
- Prostaglandins [ 0090-6980 ] ; 1995.
Abstract
The objectives of this experiment were to study the effects of pregnancy-specific protein B (PSPB) and prostaglandin E2 (PGE2) on bovine luteal cell progesterone (P4), prostaglandin F2α (PGF2α) and oxytocin production. Corpora lutea were collected during the mid- (days 10–12; n = 5) or late-luteal (days 17–18; n = 5) stages of the estrous cycle. Luteal cells were dispersed and accessory cells removed. Luteal cells (1.5 × 105) were incubated in a 3 × 3 factorial arrangement and treated with PSPB (0, 2.5, or 5.0 μg) and PGE2 (0, 100, or 200 ng) in 500 μL of Ham's F-12 medium. All cells were incubated for 18 h before adding treatments. Samples were then collected at 6 h and 12 h. During the 18 h pretreatment period, P4, PGF2α, and oxytocin production was similar between the prospective treatment groups. The PSPB failed to increase P4 production. The PGE2 × time interaction showed that P4 increased in response to PGE2 treatment at 6 h (P < 0.001) and 12 h (P < 0.03). Also, the stage × time interaction indicated that mid-stage cells produced more (P < .001) P4 than late-stage cells during the pretreatment period at 6 h and 12 h. The PSPB did not alter PGF2α production by mid-stage cells, but increased (P < .05) PGF2α by late-stage cells. Also, PGE2 stimulated (P < 0.001) PGF2α secretion by both mid- and late-stage cells; luteal cells treated with 200 ng of PGE2 produced more (P < 0.001) PGF2α than 100 ng of PGE2. Oxytocin secretion was not changed by treatment with PGE2 or PSPB. Oxytocin production was greater (P < 0.001) by mid-stage than late-stage cells during the pretreatment period at 6 h and 12 h. Oxytocin production was similar between the 6h and 12 h culture times within stage of the cycle. These data indicate that PSPB does bot change bovine luteal cell P4 or oxytocin production, but elevates PGF2α in late-stage cells. The PGF2 increases both P4 and PGF2α, but does not alter oxytocin production. Lastly, PSPB and PGE2 do not interact to promote P4, PGF2α, or oxytocin production by cultured bovine luteal cells.
Url:
DOI: 10.1016/0090-6980(95)00108-5
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Prostaglandin F2α, progesterone and oxytocin production by cultured bovine luteal cells treated with prostaglandin E2 and pregnancy-specific protein B</title><author><name sortKey="Del Vecchio, R P" sort="Del Vecchio, R P" uniqKey="Del Vecchio R" first="R. P." last="Del Vecchio">R. P. Del Vecchio</name></author><author><name sortKey="Sutherland, W D" sort="Sutherland, W D" uniqKey="Sutherland W" first="W. D." last="Sutherland">W. D. Sutherland</name></author><author><name sortKey="Sasser, R G" sort="Sasser, R G" uniqKey="Sasser R" first="R. G." last="Sasser">R. G. Sasser</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:64496B0160847331452CC0CA78CB1357F97D6E65</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0090-6980(95)00108-5</idno><idno type="url">https://api-v5.istex.fr/document/64496B0160847331452CC0CA78CB1357F97D6E65/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000895</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000895</idno><idno type="wicri:Area/Istex/Curation">000895</idno><idno type="wicri:Area/Istex/Checkpoint">001925</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001925</idno><idno type="wicri:doubleKey">0090-6980:1995:Del Vecchio R:prostaglandin:f:progesterone</idno><idno type="wicri:Area/Main/Merge">004673</idno><idno type="wicri:Area/Main/Curation">003F51</idno><idno type="wicri:Area/Main/Exploration">003F51</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Prostaglandin F2α, progesterone and oxytocin production by cultured bovine luteal cells treated with prostaglandin E2 and pregnancy-specific protein B</title><author><name sortKey="Del Vecchio, R P" sort="Del Vecchio, R P" uniqKey="Del Vecchio R" first="R. P." last="Del Vecchio">R. P. Del Vecchio</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Agriculture & Agri-Food Canada Research Centre, Brandon, Manitoba</wicri:regionArea><wicri:noRegion>Manitoba</wicri:noRegion></affiliation></author><author><name sortKey="Sutherland, W D" sort="Sutherland, W D" uniqKey="Sutherland W" first="W. D." last="Sutherland">W. D. Sutherland</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Agriculture & Agri-Food Canada Research Centre, Brandon, Manitoba</wicri:regionArea><wicri:noRegion>Manitoba</wicri:noRegion></affiliation></author><author><name sortKey="Sasser, R G" sort="Sasser, R G" uniqKey="Sasser R" first="R. G." last="Sasser">R. G. Sasser</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho</wicri:regionArea><placeName><region type="state">Idaho</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Prostaglandins</title><title level="j" type="abbrev">PROOLD</title><idno type="ISSN">0090-6980</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="137">137</biblScope><biblScope unit="page" to="150">150</biblScope></imprint><idno type="ISSN">0090-6980</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0090-6980</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objectives of this experiment were to study the effects of pregnancy-specific protein B (PSPB) and prostaglandin E2 (PGE2) on bovine luteal cell progesterone (P4), prostaglandin F2α (PGF2α) and oxytocin production. Corpora lutea were collected during the mid- (days 10–12; n = 5) or late-luteal (days 17–18; n = 5) stages of the estrous cycle. Luteal cells were dispersed and accessory cells removed. Luteal cells (1.5 × 105) were incubated in a 3 × 3 factorial arrangement and treated with PSPB (0, 2.5, or 5.0 μg) and PGE2 (0, 100, or 200 ng) in 500 μL of Ham's F-12 medium. All cells were incubated for 18 h before adding treatments. Samples were then collected at 6 h and 12 h. During the 18 h pretreatment period, P4, PGF2α, and oxytocin production was similar between the prospective treatment groups. The PSPB failed to increase P4 production. The PGE2 × time interaction showed that P4 increased in response to PGE2 treatment at 6 h (P < 0.001) and 12 h (P < 0.03). Also, the stage × time interaction indicated that mid-stage cells produced more (P < .001) P4 than late-stage cells during the pretreatment period at 6 h and 12 h. The PSPB did not alter PGF2α production by mid-stage cells, but increased (P < .05) PGF2α by late-stage cells. Also, PGE2 stimulated (P < 0.001) PGF2α secretion by both mid- and late-stage cells; luteal cells treated with 200 ng of PGE2 produced more (P < 0.001) PGF2α than 100 ng of PGE2. Oxytocin secretion was not changed by treatment with PGE2 or PSPB. Oxytocin production was greater (P < 0.001) by mid-stage than late-stage cells during the pretreatment period at 6 h and 12 h. Oxytocin production was similar between the 6h and 12 h culture times within stage of the cycle. These data indicate that PSPB does bot change bovine luteal cell P4 or oxytocin production, but elevates PGF2α in late-stage cells. The PGF2 increases both P4 and PGF2α, but does not alter oxytocin production. Lastly, PSPB and PGE2 do not interact to promote P4, PGF2α, or oxytocin production by cultured bovine luteal cells.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Idaho</li></region></list><tree><country name="Canada"><noRegion><name sortKey="Del Vecchio, R P" sort="Del Vecchio, R P" uniqKey="Del Vecchio R" first="R. P." last="Del Vecchio">R. P. Del Vecchio</name></noRegion><name sortKey="Sutherland, W D" sort="Sutherland, W D" uniqKey="Sutherland W" first="W. D." last="Sutherland">W. D. Sutherland</name></country><country name="États-Unis"><region name="Idaho"><name sortKey="Sasser, R G" sort="Sasser, R G" uniqKey="Sasser R" first="R. G." last="Sasser">R. G. Sasser</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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